The win was the first of the season for Winnipeg.
Mark Scheifele added a goal and an assist.
Josh Morrissey had a pair of assists for the Jets.
Connor Hellebuyck made 29 saves for Winnipeg, who evened their record at 1-1-0.
Evan Rodrigues two goals and an assist for the Panthers.
Sam Reinhart had a goal and an assist.
Carter Verhaeghe scored the other Florida goal.
Sergei Bobrovsky turned aside 29 shots for the Panthers, who dropped to 0-2-0 on the season.
Mark Scheifele got the Jets on the board 3:30 into the first on the power play when he took a Kyle Connor feed just inside the Panthers blue line before splitting the Panthers defence before beating Bobrovsky with a high wrister.
The goal was his second of the season.
With Brendan Dillon off for delay of game, Sam Reinhart tied the contest just 33 seconds into the ensuing power play at 17:03 when he tapped a puck past Hellebuyck at the goalmouth off a Evan Rodrigues feed from the high slot.
The goal was his first of the season.
Florida took the lead 25 seconds into the second period when Evan Rodrigues knocked a rebound off a Dmitry Kulikov point shot past Hellebuyck.
The goal was his first of the season.
Kyle Connor replied ten seconds later when he fired a Mark Scheifele feed from behind the net past Bobrovsky to knot the game at 2 at 35 seconds.
The goal was his second of the season.
Morgan Barron scored 8:20 into the second to give Winnipeg the lead when Rasmus Kupari got hold of the puck deep in the Florida end before working his way behind the the Panthers goal before circling back on his backhand before flipping Barron the puck.
Barron simply slipped the disc into the goal.
Dylan DeMelo made it 4-2 when he took a Cole Perfetti feed from the left corner and ripped a wrister from the high slot past Bobrovsky, beating him high blocker side to score his first of the season at 9:55.
Mason Appleton got Winnipeg's fifth goal of the game shorthanded at 14:17 of the third after converting a Lowry centering pass.
Lowry had the stick knocked out of his hands in the Jet zone before he kicked the disc ahead and had a stick handed to him by a teammate off the bench.
Lowry skated into the Florida end before feeding Appleton who kneaded a forehand through Bobrovsky's pads to sscore his first of the season.
Carter Verhaeghe scored with 3:29 remaining in the third to pull the Panthers to within two after Paul Maurice pulled his goaltender.
Aleksander Barkov scored at 17:36 after Sam Reinhart pulled up just over the Winnipeg blue line and hit him with a crisp pass.
Barkov beat Hellebuyck with a forty foot wrister to make it 5-4.
Tkachuk nearly tied it with about a minute and a half left in regulation when he hit the goalpost on a partial breakaway.
Kyle Connor added an empty netter with 1:10 to go to seal the win for the Jets, their first of the regular season.
Florida visits New Jersey Monday while Winnipeg hosts Los Angeles Tuesday.
SHOTS-FLORIDA 33 WINNIPEG 35
NOTES-The game was Jet defenceman Nate Schmidt's 600th NHL contest.
